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anr 94  (Tocris)


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    Tocris anr 94
    Anr 94, supplied by Tocris, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anr+94/ANR+94/pmc07069424-38-11-50
    Average 90 stars, based on 3 article reviews
    anr 94 - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Differential effect of adenosine on rhabdomyosarcoma migration and proliferation
    Article Snippet: PD 184352 (2-[(2-chloro-4-iodophenyl)amino]- N -cyclopropylmethoxy)-3,4-difluorobenzamide), U 73122 (1-[6-[[(17β)-3-methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-1 H -pyrrole -2,5-dione), ANR 94 (8-ethoxy-9-ethyl-9 H -purin-6-amine), PSB 603 (8-[4-[4-(4-Chloro phenzyl)piperazide-1-sulfonyl)phenyl]]-1-propylxanthine), MRS 1220 ( N -[9-chloro-2-(2-fura nyl)[ , , ]-triazolo[1,5- c ]quinazolin-5-yl]benzene acetamide), pertussis toxin (50 ng/ml), IB-MECA (1-deoxy-1-[6-[[(3-iodophenyl)methyl]amino]-9 H -purin-9-yl]- N -methyl-β-D-ribofuranuronamide), and CPA ( N- cyclopentyladenosine) were purchased from Tocris (Ellisville, MO).



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    Effects of adenosine and the A2A adenosine receptor antagonists SCH58261 (SCH) and <t>ANR94</t> (ANR) on T-cell chemotaxis. T cells from 2 older men and women (n=4) and 2 younger matched control men and women were preincubated for 15 min and then continuously with 0.1, 0.3, or 1.0 μM adenosine, 10 μM EHNA, or 20 nM SCH58261 for 4 h of chemotaxis to 50 nM CCL21 or 100 nM S1P. T cells from 2 older men and 1 old woman (n=3) and 2 younger matched control men and 1 woman similarly were preincubated for 15 min and then continuously with 460 nM ANR94 for 4 h of chemotaxis to 50 nM CCL21 or 100 nM S1P. Bars represent means ± sd of each cluster of responses as a percentage of the control responses (100%). Mean CCL21 control chemotactic responses for adenosine, EHNA, and SCH series were 24.6 ± 7.1% of initial T cells for younger donors and 16.7 ± 3.8% of initial T cells for older donors (69% of young control group level). Mean CCL21 control chemotactic responses for ANR series were 31.8 ± 6.5% of initial T cells for younger donors and 24.7 ± 3.5% of initial T cells for older donors (68% of young control group level). Mean S1P control chemotactic responses for SCH and ANR series were 7.2 ± 1.1% of initial T cells for younger donors and 5.3 ± 0.6% of initial T cells for older donors (74% of young control group level). Symbols at tops of bars indicate statistically significant difference vs. corresponding control; symbols next to horizontal arrowheads indicate difference between bars of that set. +P < 0.05, *P < 0.01, #P < 0.01.
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    Effects of adenosine and the A2A adenosine receptor antagonists SCH58261 (SCH) and <t>ANR94</t> (ANR) on T-cell chemotaxis. T cells from 2 older men and women (n=4) and 2 younger matched control men and women were preincubated for 15 min and then continuously with 0.1, 0.3, or 1.0 μM adenosine, 10 μM EHNA, or 20 nM SCH58261 for 4 h of chemotaxis to 50 nM CCL21 or 100 nM S1P. T cells from 2 older men and 1 old woman (n=3) and 2 younger matched control men and 1 woman similarly were preincubated for 15 min and then continuously with 460 nM ANR94 for 4 h of chemotaxis to 50 nM CCL21 or 100 nM S1P. Bars represent means ± sd of each cluster of responses as a percentage of the control responses (100%). Mean CCL21 control chemotactic responses for adenosine, EHNA, and SCH series were 24.6 ± 7.1% of initial T cells for younger donors and 16.7 ± 3.8% of initial T cells for older donors (69% of young control group level). Mean CCL21 control chemotactic responses for ANR series were 31.8 ± 6.5% of initial T cells for younger donors and 24.7 ± 3.5% of initial T cells for older donors (68% of young control group level). Mean S1P control chemotactic responses for SCH and ANR series were 7.2 ± 1.1% of initial T cells for younger donors and 5.3 ± 0.6% of initial T cells for older donors (74% of young control group level). Symbols at tops of bars indicate statistically significant difference vs. corresponding control; symbols next to horizontal arrowheads indicate difference between bars of that set. +P < 0.05, *P < 0.01, #P < 0.01.
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    Effects of adenosine and the A2A adenosine receptor antagonists SCH58261 (SCH) and ANR94 (ANR) on T-cell chemotaxis. T cells from 2 older men and women (n=4) and 2 younger matched control men and women were preincubated for 15 min and then continuously with 0.1, 0.3, or 1.0 μM adenosine, 10 μM EHNA, or 20 nM SCH58261 for 4 h of chemotaxis to 50 nM CCL21 or 100 nM S1P. T cells from 2 older men and 1 old woman (n=3) and 2 younger matched control men and 1 woman similarly were preincubated for 15 min and then continuously with 460 nM ANR94 for 4 h of chemotaxis to 50 nM CCL21 or 100 nM S1P. Bars represent means ± sd of each cluster of responses as a percentage of the control responses (100%). Mean CCL21 control chemotactic responses for adenosine, EHNA, and SCH series were 24.6 ± 7.1% of initial T cells for younger donors and 16.7 ± 3.8% of initial T cells for older donors (69% of young control group level). Mean CCL21 control chemotactic responses for ANR series were 31.8 ± 6.5% of initial T cells for younger donors and 24.7 ± 3.5% of initial T cells for older donors (68% of young control group level). Mean S1P control chemotactic responses for SCH and ANR series were 7.2 ± 1.1% of initial T cells for younger donors and 5.3 ± 0.6% of initial T cells for older donors (74% of young control group level). Symbols at tops of bars indicate statistically significant difference vs. corresponding control; symbols next to horizontal arrowheads indicate difference between bars of that set. +P < 0.05, *P < 0.01, #P < 0.01.

    Journal: The FASEB Journal

    Article Title: Distinctive immunoregulatory effects of adenosine on T cells of older humans

    doi: 10.1096/fj.11-197046

    Figure Lengend Snippet: Effects of adenosine and the A2A adenosine receptor antagonists SCH58261 (SCH) and ANR94 (ANR) on T-cell chemotaxis. T cells from 2 older men and women (n=4) and 2 younger matched control men and women were preincubated for 15 min and then continuously with 0.1, 0.3, or 1.0 μM adenosine, 10 μM EHNA, or 20 nM SCH58261 for 4 h of chemotaxis to 50 nM CCL21 or 100 nM S1P. T cells from 2 older men and 1 old woman (n=3) and 2 younger matched control men and 1 woman similarly were preincubated for 15 min and then continuously with 460 nM ANR94 for 4 h of chemotaxis to 50 nM CCL21 or 100 nM S1P. Bars represent means ± sd of each cluster of responses as a percentage of the control responses (100%). Mean CCL21 control chemotactic responses for adenosine, EHNA, and SCH series were 24.6 ± 7.1% of initial T cells for younger donors and 16.7 ± 3.8% of initial T cells for older donors (69% of young control group level). Mean CCL21 control chemotactic responses for ANR series were 31.8 ± 6.5% of initial T cells for younger donors and 24.7 ± 3.5% of initial T cells for older donors (68% of young control group level). Mean S1P control chemotactic responses for SCH and ANR series were 7.2 ± 1.1% of initial T cells for younger donors and 5.3 ± 0.6% of initial T cells for older donors (74% of young control group level). Symbols at tops of bars indicate statistically significant difference vs. corresponding control; symbols next to horizontal arrowheads indicate difference between bars of that set. +P < 0.05, *P < 0.01, #P < 0.01.

    Article Snippet: The A 2A receptor antagonists ANR94 and SCH58261 and the A 2A receptor agonist CGS21680 hydrochloride (Tocris Biosciences, Ellisville, MO, USA), the A 3 receptor antagonist 2-phenyl-amino-N6-endo-norbornyladenine (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the ADA inhibitor erythro-9-(2-hydroxy-3-nonyl)-adenine hydrochloride (EHNA), adenosine, sphingosine 1-phosphate (S1P), and recrystallized bovine serum albumin (Sigma Chemicals, St. Louis, MO, USA) were obtained from the designated suppliers.

    Techniques: Chemotaxis Assay, Control

    Effects of adenosine and the A2A adenosine receptor antagonists SCH58261 (SCH) and ANR94 (ANR) on T-cell chemotaxis. T cells from 2 older men and women (n=4) and 2 younger matched control men and women were preincubated for 15 min and then continuously with 0.1, 0.3, or 1.0 μM adenosine, 10 μM EHNA, or 20 nM SCH58261 for 4 h of chemotaxis to 50 nM CCL21 or 100 nM S1P. T cells from 2 older men and 1 old woman (n=3) and 2 younger matched control men and 1 woman similarly were preincubated for 15 min and then continuously with 460 nM ANR94 for 4 h of chemotaxis to 50 nM CCL21 or 100 nM S1P. Bars represent means ± sd of each cluster of responses as a percentage of the control responses (100%). Mean CCL21 control chemotactic responses for adenosine, EHNA, and SCH series were 24.6 ± 7.1% of initial T cells for younger donors and 16.7 ± 3.8% of initial T cells for older donors (69% of young control group level). Mean CCL21 control chemotactic responses for ANR series were 31.8 ± 6.5% of initial T cells for younger donors and 24.7 ± 3.5% of initial T cells for older donors (68% of young control group level). Mean S1P control chemotactic responses for SCH and ANR series were 7.2 ± 1.1% of initial T cells for younger donors and 5.3 ± 0.6% of initial T cells for older donors (74% of young control group level). Symbols at tops of bars indicate statistically significant difference vs. corresponding control; symbols next to horizontal arrowheads indicate difference between bars of that set. +P < 0.05, *P < 0.01, #P < 0.01.

    Journal: The FASEB Journal

    Article Title: Distinctive immunoregulatory effects of adenosine on T cells of older humans

    doi: 10.1096/fj.11-197046

    Figure Lengend Snippet: Effects of adenosine and the A2A adenosine receptor antagonists SCH58261 (SCH) and ANR94 (ANR) on T-cell chemotaxis. T cells from 2 older men and women (n=4) and 2 younger matched control men and women were preincubated for 15 min and then continuously with 0.1, 0.3, or 1.0 μM adenosine, 10 μM EHNA, or 20 nM SCH58261 for 4 h of chemotaxis to 50 nM CCL21 or 100 nM S1P. T cells from 2 older men and 1 old woman (n=3) and 2 younger matched control men and 1 woman similarly were preincubated for 15 min and then continuously with 460 nM ANR94 for 4 h of chemotaxis to 50 nM CCL21 or 100 nM S1P. Bars represent means ± sd of each cluster of responses as a percentage of the control responses (100%). Mean CCL21 control chemotactic responses for adenosine, EHNA, and SCH series were 24.6 ± 7.1% of initial T cells for younger donors and 16.7 ± 3.8% of initial T cells for older donors (69% of young control group level). Mean CCL21 control chemotactic responses for ANR series were 31.8 ± 6.5% of initial T cells for younger donors and 24.7 ± 3.5% of initial T cells for older donors (68% of young control group level). Mean S1P control chemotactic responses for SCH and ANR series were 7.2 ± 1.1% of initial T cells for younger donors and 5.3 ± 0.6% of initial T cells for older donors (74% of young control group level). Symbols at tops of bars indicate statistically significant difference vs. corresponding control; symbols next to horizontal arrowheads indicate difference between bars of that set. +P < 0.05, *P < 0.01, #P < 0.01.

    Article Snippet: Chemicals The A 2A receptor antagonists ANR94 and SCH58261 and the A 2A receptor agonist CGS21680 hydrochloride (Tocris Biosciences, Ellisville, MO, USA), the A 3 receptor antagonist 2-phenyl-amino-N6-endo-norbornyladenine (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the ADA inhibitor erythro-9-(2-hydroxy-3-nonyl)-adenine hydrochloride (EHNA), adenosine, sphingosine 1-phosphate (S1P), and recrystallized bovine serum albumin (Sigma Chemicals, St. Louis, MO, USA) were obtained from the designated suppliers.

    Techniques: Chemotaxis Assay, Control